KMID : 0665220150280030369
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Korean Journal of Food and Nutrition 2015 Volume.28 No. 3 p.369 ~ p.375
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Physiological Activities of Ginkgo biloba Sarcotesta Extract with Heat Treatment
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Kim Sung-Tae
Lee Ji-Hyun Lee Sang-Hoon Jang Gwi-Yeong Kim Min-Young Yoon Na-ra Lee Jun-Soo Jeong Heon-Sang
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Abstract
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This study was performed to investigate the physiological activities of Ginkgo biloba sarcotesta extracts before and after heat treatment. G. biloba sarcotesta was heated at 130¡É for 2 h and extracted with water, 70% ethanol and 80% methanol. ABTS and DPPH radical scavenging activities increased after heating in the water (14.95 mg AAE/g and 7.36 mg TE/g) and ethanol extracts (12.20 mg AAE/g and 6.23 mg TE/g). ¥á-Glucosidase inhibitory activity decreased after heating in all but the water extract. Angiotensin converting enzyme I inhibitory activities decreased after heating in all extracts. Nitric oxide production inhibitory activity increased from 12.40~44.55% of the raw sample to 40.76~72.39% of the heated sample at a concentration of 200 ¥ìg/mL. Lipid accumulation inhibitory activities were similar before and after heat treatment. The highest antiproliferative effects on MCF-7 human breast cancer cell lines were observed in 80% methanol extract in the heated sample. Cell viability at concentrations of 25, 50, 100, and 200 ¥ìg/mL measured 34.88, 17.58, 8.44 and 10.48%, respectively. From the results, the antioxidant and antiproliferative activities of G. biloba sarcotesta extracts increased with heat treatment, and research on the identification of the structure for the active compounds are needed in further studies.
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KEYWORD
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Ginkgo biloba sarcotesta , heat treatment , physiological activity
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